Mathieu Bennet: In B-cell cloning, single-cell isolation occurs at two crucial stages. First, during the initial screening phase, high-throughput isolation is required to analyze a broad antibody ...

(A) The RNA-overhang cloning method. The parental genes (top) are the human AMPA glutamate receptor GluR2 intron 14 and its flanking exons (this gene is shown twice: lines 1 and 3), and the human ...