Here, we used acute rat hippocampal slices to analyze the mechanisms underlying rapid changes in mTOR activity and actin polymerization elicited by estradiol. Estradiol-induced mTOR phosphorylation ...

(B) GST-Mlph-ABD was incubated with actin in the presence of different concentrations of Flag-Myo5a-MTD and ... Data are the mean ± SD of three independent experiments with one-way ANOVA with post-hoc ...

Western Blot 即免疫印迹试验，可用来比较在不同条件下 ... 内参抗体种类很多，比如β-actin、β-tubunlin、GAPDH、Lamin B等，那么针对自己的实验，我们该如何选择呢?下面简单介绍下： 一．样本种属来源： 首先要考虑的就是实验样本来源于什么物种。

Scale bar: 3 µm. Total actin expression levels shown by western blotting for diploid strains expressing one or two actin variants, with tubulin (Tub1p) as a loading control. B. Comparison of actin ...

A western blot, sometimes called a protein immunoblot, is an antibody-based technique used to detect the presence, size and abundance of specific proteins within a sample. The technique was developed ...

再Western Blotting中使用内参其实就是再WB过程中的另外用内参对应 ... (1)哺乳动物的组织或者细胞样本，通常选择β-actin、β-tubulin、GAPDH、Lamin B、Histone H3、Na,Katpase等。 (2)植物来源实验样本，则可以选择plant actin、Rubisco等。 (3)其他来源样本研究较少，所以就应该 ...

Western bolting is a well-established method that is currently relied on in biology laboratories for determining the specific proteins and their abundance within a given sample of cells or tissue.