The quality of qPCR and RT-qPCR results can be negatively affected by many experimental variables. To ensure the validity of assay results, sample extraction and preparation steps must be closely ...

However, the accuracy and reproducibility of qPCR results are heavily influenced by the proper use of reference genes as controls. These controls are essential for ensuring that the results reflect ...

Download this article from Bio-Rad to learn tips and tricks for better assay control management and superior qPCR results. Is the form not loading? If you use an ad blocker or browser privacy features ...

4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 These guidelines ...

From detecting trace levels of virus to quantifying oncogenes in patient samples, quantitative PCR (qPCR) is the cornerstone of many diagnostic tests. At the basic research-level, scientists rely on ...