If only one gene in the sample is being interrogated, a gene-specific primer can yield good results. Otherwise, the best qPCR results came from combinations of oligo(T)s (targeting the poly(A ...

4. When reporting qPCR results, review the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, as described by Bustin, et al. 3 These guidelines ...

The quality of qPCR and RT-qPCR results can be negatively affected by many experimental variables. To ensure the validity of assay results, sample extraction and preparation steps must be closely ...

However, the accuracy and reproducibility of qPCR results are heavily influenced by the proper use of reference genes as controls. These controls are essential for ensuring that the results reflect ...

Analyzing results from qPCR centers on an amplification plot, which charts the cycle number against the fluorescent signal. Initial qPCR cycles with minimal amplification and relatively low ...