qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

Antibiotic resistance is a global concern that threatens our ability to prevent and treat bacterial infections in humans and animals. To better monitor the emergence and spread of resistance, ...

Antibiotic resistance is a global concern that threatens our ability to prevent and treat bacterial infections in humans and animals. To better monitor the emergence and spread of resistance, ...

With laboratory methods and diagnostics advancing apace, Francesca Lake considers whether PCR is likely to retain its crown as an essential lab method, or whether it could be replaced and consigned to ...

Here’s a handy overview of the technique, how it works and a few refresher details on viruses and genetics. What is real time RT–PCR? Real time RT–PCR is a nuclear-derived method for detecting the ...

Additional variations to the cycling conditions or PCR mix may also help with detection. 5 This method is particularly useful for detecting trace amounts of DNA, which makes it applicable in forensic ...

The GoTaq ® Probe 1-Step RT-qPCR System enables detection and relative quantification of RNA expression levels using a one-step RT-qPCR method, combining GoScript™ Reverse Transcriptase and ...

A laboratory practical illustrating the use of the ChIP-qPCR method in a robust model: Estrogen receptor alpha immunoprecipitation using Mcf-7 culture cells.