该研究团队设计了基于热动力学的基因特异qPCR引物设计流程，并对人、小鼠、家蚕、斑马鱼、线虫、酵母、拟南芥、水稻、玉米和油菜等共147个已 ...

然而，各种PCR技术，包括PCR、qPCR、RT-PCR、RT-qPCR、Real-Time PCR和dPCR，长得实在是太像了，好多同学看得头晕眼花也没弄明白这几种PCR有什么区别。

为什么更换仪器后会出现BadRox报错？ BadRox报错是因为更换qPCR仪器后，试剂中添加的ROX浓度与仪器不匹配导致的。 什么是ROX？ ROX是一种惰性参比荧光 ...

To calculate the copy number of the blinded and macaque samples, the data generated by nested qPCR was linearly fitted with the linear equation obtained from the LOD/LOQ standard curve for each of ...

The R 2 value is the coefficient of correlation obtained for the standard curve, indicating how good one value is at predicting the other and should be as close to 1 as possible, at least > 0.99.

She is passionate about making complex scientific concepts accessible to readers. View Full Profile. Learn about our Editorial Policies. Quantitative polymerase chain reaction (qPCR) is a molecular ...

Quantitative PCR (qPCR) and digital PCR (dPCR) are powerful tools for nucleic acid detection and quantification, each with distinct strengths. Both technologies play vital roles in genomics research, ...

EvaGreen® is less likely to inhibit PCR and promote mispriming than competitor DNA-binding dyes, and it shows superior performance when used in qPCR and for high resolution melt curve analysis. This ...

31 October 2024, Birmingham UK - Precision genomics company, Nonacus Ltd, has launched GALEAS TM uPCR ESR1, an ultrasensitive qPCR (uPCR) assay developed to detect eleven mutations in the ESR1 gene ...