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We developed a methodology to measure local protein synthesis by puromycin labeling of isolated synaptosomes devoid of somatic input. Our results show that synaptic local translation is enhanced or ...

听说有人药死过自己的细胞？在细胞实验药筛过程中往往会因为药筛浓度、药筛时间等因素导致细胞活活被药死。 药筛抗生素在筛选稳转细胞株以及基因编辑转染实验中会经常用到，它们能够杀死未转染或未整合抗性基因的细胞，从而使得带有抗性基因的细胞 ...

Two PPP2R3A short hairpin RNA (shRNA) lentiviral vectors and a negative control vector were purchased from GenePharma and transfected into HEK293T cells using lipofectamine 3000 to generate and ...

Cells were plated at a density of 3×10 5 cells/well in 6-well plates and grown until 80% confluence. Cells were then transfected with 4 μg of plasmid per well. Puromycin was added to medium to a final ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Stimulator of interferon genes (STING) is ...

在生命的微观剧场中，RNA分子如同精准的时空指挥官，通过动态的定位与运输调控基因表达、蛋白质合成乃至细胞命运。然而，研究人员长久以来 ...