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Here, authors use cryo-EM to capture five unique states that show how a mobile catalytic Mg 2+ drives phosphodiester bond formation.

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DNA is a chemical polymer that is unstable in the presence of water because of its sensitivity to the addition of water across its phosphodiester bonds, which produces strand breakage 1.

This landmark study describes the structure of the human RAD51 filament with a recombination intermediate called the displacement loop (D-loop). Using cryogenic structural, biochemical, and ...