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核移植胚胎重编程的细胞特异性差异及关键影响因素探究
二、研究方法 实验动物与胚胎处理:使用非洲爪蟾(Xenopus laevis)进行实验 ... 通过注射 mRNA 和吗啉代寡核苷酸(morpholino oligo),分别过表达和抑制特定基因,观察对胚胎发育的影响。 其他实验方法:通过免疫组织化学染色检测 Tp63 蛋白,评估基底干细胞 ...
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