Here, we present a replication-competent Sindbis viral vector that has an hsvtk suicide gene incorporated into ns3, ... Wild-type Sindbis virus RNA genome has two major groups of genes: ...

and Jeanne M. Rowe Center for Research in Virology at the Morgridge Institute and professor of oncology and molecular virology at UW–Madison, first revealed the existence of this crown-like viral RNA ...

The scientists have uncovered that certain RNA viruses take control of a cellular enzyme to design a replication compartment on the cell's membrane filled with PI4P lipids. Those lipids ...

Such a response can be weaponized against malignant cells by modifying T cells with DNA or RNA coding ... chances that replication competent viruses might be generated during vector production.

Researchers offer new insights on how subunits of the influenza virus polymerase co-evolve to ensure efficient viral RNA replication. New insights on how subunits of the influenza virus polymerase ...

The viral RNA replication protein that forms the crown is an extremely large, multi-domain, multi-functional protein, nearly 1000 amino acids in size.

Within an infected cell, viral RNA replication occurs at modified cellular membranes, often in association with spherules, virus-induced vesicles approximately 50–100 nanometers in size.

In a recent study posted to bioRxiv*, researchers evaluated the early replication kinetics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Study: Visualization of early RNA ...

The authors developed a non-transgenic genome editing approach in tobacco by delivering CRISPR/Cas9 with an engineered eggplant mottled dwarf virus (EMDV) vector, a negative-strand RNA virus ...

It is also why developing highly effective vaccines against RNA viruses remains challenging. A second, indirect consequence of the low-fidelity replication process is the small size of the viral ...

Lentiviruses are characterized by an envelope, capsid and a RNA genome. The lentiviral vector was engineered so it did not contain any viral-coding genes whilst maintaining genes for replication and ...