Electroporation works well with cell lines that are refractive to other techniques, such as calcium phosphate–DNA coprecipitation. But as with other transfection methods, the optimal conditions ...

Change the medium 1 h before transfection. 2| To prepare the calcium phosphate–DNA coprecipitate, combine 100 μl of 2.5 M CaCl 2 with 25 μg of plasmid DNA in a sterile 5-ml plastic tube and ...

The most recent and sophisticated multi-component transfection reagent, FuGENE ® 4K, was created to transfer DNA into both difficult and routine mammalian cell lines. Users are able to obtain ...

操作步骤简单：简单易用的操作步骤，可进行反向转染 (Reverse Transfection)，无需复杂优化。 在转染前一天铺细胞，在转染当天 细胞融合度在75%左右。 转染当天，将以无血清培养基稀释 DNA。再加入适量ViaFect™ 转染试 剂与DNA构成适当比例，孵育 5-20min。 加入适量 ...

Beginning at cloning or transfection requires more interaction between the VDL and you. Briefly, once cells have been transfected, an agar overlay is applied to the cells so that virus-producing cells ...

Reporter genes are generally joined to a regulatory DNA sequence in an expression vector that is usually propagated in Escherichia coli before transfection into the cell type of interest. 1 A control ...