As shown in Figure 2, the relative magnitude of the equilibrium constant for a reaction with a 1:1 stoichiometry affects the shape of the titration curve, as does the concentrations of the ...

Along with that, we saw that the MW trace reached a complex with 2:1 Nap1:H2A-H2B stoichiometry. Upon further titration, beyond an equimolar amount (1.5 and 2.0 molar equivalents), the MW trace ...

Also, knowing the concentrations of the two components, for instance protein and ligand, makes it possible to determine the stoichiometry of the binding reaction. If the titration is performed at ...

The titration is performed at constant pressure and temperature, meaning that a single ITC experiment offers data on the binding enthalpy, the equilibrium association constant and the stoichiometry, ...

Isothermal titration calorimetry (ITC ... macromolecule inside the cell is 2–3 to ensure near saturation (for 1:1 stoichiometry). Considering the volume of the sample cell (1.4 ml), the typical ...

Automated washing with detergent of the sample cell and titration syringe assists in producing high quality reproducible data. • All binding parameters (affinity, stoichiometry, enthalpy and entropy) ...

In his honor the titration is named Karl Fischer titration (KF titration for short). In the following years both original stoichiometry and reagents were revised. Fischer presented the reaction which ...

Find example text on the Acknowledgement Page or the individual instrument pages. Isothermal Titration Calorimetry (ITC) is a widely utilised technique in biochemistry and biophysics for ...