We demonstrate that the single hydroxyl group of the conserved threonine is essential to maintain remarkably high suppression of side reactions, but has only marginal effects on the catalytic rate ...

Figure 4: The threonine-phosphatase activity of EYA4 is required for the innate immune reaction. For recombinant EYA, the Flag-tag was ligated at the 5′ end of Eya, and inserted into the pEF-BOS ...