We demonstrate that the single hydroxyl group of the conserved threonine is essential to maintain remarkably high suppression of side reactions, but has only marginal effects on the catalytic rate ...

Cisbio's HTRF® KinEASE™ assay for screening serine/threonine kinases now extends ... The assay is run in two main steps, the kinase reaction step followed by the detection step with HTRF ...

The original reaction is as below: The KF titration reaction currently accepted is as follows, and was reached by several advances in understanding the mechanism of the reaction and modification ...