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The viability was measured using a standard CCK8 assay (Promega, Beijing, China). Real-Time PCR Analysis of Secreted Protein, Acidic And Rich In Cysteine Expression Total RNA of MDA-MB-231 cells and ...

Takara Shuzo (Otsu, Japan) developed both ELISA and western blot analyses for detecting antibodies against CH-296, and transferred the standard operating procedure to Tanox (Houston, Texas).

From the extract, 1 μg of mRNA was reverse-transcribed into cDNA (SensiFAST cDNA Synthesis Kit; Meridian Life Science, Inc., Memphis, TN, USA) and amplified with the SYBR green PCR master mix (Takara ...