During the past few years a novel approach based on the use of type IIS restriction enzymes in combination with T4 DNA ligase has become popular (known as Golden Gate cloning, PLoS ONE 3 ...

New England Biolabs (NEB®) announces the introduction of the NEBuilder HiFi DNA Assembly Cloning Kit and Master Mix for one-step cloning and multiple DNA fragment assembly in as little as 15 minutes.

"The barrier of cloning primate species is now overcome ... dilemma" over whether to adapt it for humans. The key step of transferring DNA might be combined with gene editing to correct genetic ...

In a highly unusual step, and in reaction to the South Korean fraud, an independent team of scientists in Australia verified that the Oregon team had indeed produced stem cells through cloning.

Each cycle of PCR has three main steps, as described in the following ... Once PCR cycling is complete, the copied DNA molecules can be used for cloning, sequencing, mapping mutations, or studying ...

But whether or not they realize it, many thousands of clones have already been produced as the cloning process has become ...

Dr. Charles Strom, director of the Genetics and DNA Laboratory at ... the U.S. should take further steps against such research. Asked about the pros and cons of cloning, commission member ...

Furthermore, the sequencing was accomplished using long sequencing reads without the need for any DNA amplification techniques—an essential step for ... artifacts that cloning can introduce.” ...

Like a storyline pulled from the movie "Jurassic Park," a professor at Harvard University has outlined steps for cloning a long-extinct Neanderthal baby. One's first question may be, "Why would ...

The cloning of long DNA segments, especially those containing ... sequences of up to 100 kb to be accomplished in a single step. The target genome segment is excised from bacterial chromosomes ...