We isolated total RNA from lymphoblastoid cells of four MUL patients, one MUL carrier and one control individual using the RNeasy RNA extraction kit (Qiagen) and synthesized cDNA using M-MLV ...

Total RNA was extracted from freshly isolated airway epithelial cells, from cultured 16HBE14o− cells, and from HAE cells, after either submerged or ALI culture (HAE cells), using the RNeasy mini kit ...

Regional expression of the GI appetite hormones CCK, PYY and GCG was also determined in mouse. RNA was extracted from tissues using an RNeasy Mini kit (Qiagen). RNA quantity and quality was assessed ...