RNA-Seq enables rapid sequencing of total cellular RNA and should allow the reconstruction of spliced transcripts in a cell population. Trapnell et al. achieve this and transcript quantification ...

之前整理的一篇大综述 — Nature重磅综述 |关于RNA-seq，你想知道的都在这收到了热烈反响，阅读人数过万。 行文很长，最后精炼下来的文字近三万 ...

传统群体转录组学难以揭示细菌群落异质性。研究人员开发液滴式高通量单细胞微生物 RNA 测序方法（smRandom-seq），可实现高物种特异性和灵敏的基因检测，涵盖样本预处理、原位反应及建库等流程，为菌群异质性等研究提供新工具。

Explore the fundamentals of RNA-seq, its applications in gene expression analysis and a step-by-step protocol. ... as are quality control tools like Picard or Qualimap. 13 De novo assembly will allow ...

PolyA-RNA-seq: Capture of coding RNAs. Requires intact RNA. Full-length transcripts are captured. Recommended read depth is 15-30 million reads/sample although deeper sequencing may be required in ...

Here, we highlight a few applications of full-length RNA sequencing to better understand plant and animal genomes and the workflow for isoform sequencing methods (Figure 1). Figure 1.

Long-read sequencing technology, compared to widely-used short-read sequencing technology, can improve mapping accuracy, genome assembly, and structural variation detection.

Novel long-read sequencing data approaches are presented including tools enabling genome assembly, overcoming challenges posed by repetitive regions (Chakravarty et al. 2025; Vrček et al. 2025 ...

21 Sep 2022. As some scientists create huge, new, single-nucleus RNA-sequencing datasets, others devise ways to better use existing ones. At the Alzheimer’s Association International Conference held ...