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【卡梅德生物】噬菌体展示抗体库技术如何实现噬菌体库抗体筛选
通过基因工程技术,将编码抗体可变区(Fab 或 scFv 片段)的基因精准插入到噬菌体表面蛋白基因中。以丝状噬菌体为例,常见的是将抗体基因与 pIII 或 pVIII 蛋白基因融合。当噬菌体在大肠杆菌等宿主细胞内进行复制和组装时,抗体基因随之表达,并与噬菌体 ...
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