Of these 19 proteins, 15 had higher expression levels in the FGY217 pep4 deletion strain ... The presence of “free” GFP after overexpression of membrane protein-GFP fusions in yeast may be a result of ...

Subsequently, GP32Δ was cloned into a prokaryotic expression vector for expression and purification, resulting in the successful production ... Western blot analysis was conducted to assess their ...

In contrast, rat CRP (rCRP) is an atypical acute-phase protein that possesses unique features ... Here we explored various strategies for rCRP purification, including direct isolation from rat plasma ...

[Objective] To construct a prokaryotic expression system for monkeypox virus B20R protein and evaluate the immunogenicity of the fusion protein obtained from prokaryotic expression, to provide ...

Mouse PNAS-4 (mPNAS-4) has 96% identity with human PNAS-4 (hPNAS-4) in primary sequence and has been reported to be involved in the apoptotic response to DNA damage. However, there have been no ...

Kymera and Sanofi shared positive results for an IRAK4 degrader for hidradenitis suppurativa (HS) and atopic dermatitis (AD), breaking ground to take protein degradation outside the field of oncology ...

The envelope E2 protein is one of the promising subunit vaccine candidates against CHIKV. In this study, we describe successful cloning, expression, and purification of CHIKV E2 full-length (E2-FL ...

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