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Three PCR-negative colonies in the three-fragment Nimble Cloning with the reaction time of 5 min were sequenced, and they all were the products of self-ligation of the vector between the two SfiI ...

Subsequently, GP32Δ was cloned into a prokaryotic expression vector for expression and purification, resulting in the successful production of soluble recombinant protein rP32Δ. Utilizing rP32Δ, an ...

Breast, leg and myocardial tissue samples of 55-day-old Xingyi ducks were randomly selected for total RNA extraction, followed by cloning into pMD18-T and then constructed into the prokaryotic ...

Abstract: In order to study the LscW of Lawsonia intracellularis, the prokaryotic expression vector of LscW protein was constructed. Two primers were designed in this experiment. The 1197 bp lscW gene ...

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By Expression Systems (Prokaryotic Expression Systems, Mammalian Cell Systems, Insect Cell Systems, Yeast Systems, Cell-free Expression Systems, Others), By Products (Reagents, Competent Cells, ...

We amplified exon 9 of MEF2D gene by PCR and analyzed after sequencing. The entire CDS region was amplified by RT-PCR. The prokaryotic expression vector was constructed by double enzyme digestion.