This protocol describes how to remove the contents of a single plant cell and directly analyze the metabolites by mass spectrometry. ... LC-MS grade (Kanto Chemical, cat. no. 25185-76, CAS (67-56-1)) ...

Almost all plant cells have large vacuoles that contain both hydrolytic enzymes and a variety of defense proteins. Plants use vacuoles and vacuolar contents for programmed cell death (PCD) in two ...