The phosphate group attached to the 5' carbon of the sugar on one nucleotide forms an ester bond with the free hydroxyl on the 3' carbon of the next nucleotide.

Figure 1. Hydrolysis of an ester consists of water adding to the ester bond and displacing an alcohol (R-OH), forming a carboxylic acid. The red boxes show the original water molecule. The blue hatch ...

Where the phosphate group proximal to the nucleoside the site of the phosphate-ester bond. Structure of purines. Purine biosynthesis is complex.

Acid phosphatase hydrolyses phosphate ester bonds under an optimum pH of 4.8 and an optimum temperature of 37 °C. In this experiment you will use an artificial substrate, para-nitrophenylphosphate ...

Ester bonds are made between the fifth carbon atom in a 5-carbon sugar and the phosphate group. ... The bonds between the three phosphate groups are high energy bonds, ...