A second adapter was then ligated to the 3’OH of the cDNAs (with NEB HC RNA Ligase in NEB ligation buffer plus 5% DMSO, 1 mM ATP, and 22.5% PEG8000) at 20 °C and 1250 rpm overnight. The adapter ...

High fidelity human mitochondrial DNA polymerase (Pol γ) contains two active sites, a DNA polymerization site (pol) and a 3′-5′ exonuclease site (exo) for proofreading. Although separated by 35 Å, ...

Rolling circle amplification (RCA) is an isothermal amplification technology to produce periodic ssDNA chains, which relies on circular DNA templates and the catalysis of phi29 DNA polymerase. (41) ...

这篇综述系统介绍了DNA curtain技术如何整合全内反射荧光显微镜(TIRFM)、脂质流动性、微流控和纳米加工技术，实现对蛋白质-DNA相互作用的实时可视化。文章重点阐述了该技术在核苷酸切除修复(NER)、R-loop调控、复制-转录冲突及染色质动力学研究中的突破性应用，揭示了XPC-RAD23B的损伤搜索机制、TonEBP ...

Phi29 DNA聚合酶(Phi29 Pol)作为第三代测序技术(如DNA纳米球测序)的核心工具，其天然版本在300 mM KCl等高盐环境下扩增效率低下。 传统酶改造依赖费时费力的试错过程，而这项研究创新性地建立了微滴荧光检测系统——通过核酸凝胶染料将滚环扩增(RCA)效率转化为荧光信号，首次实现Phi29 Pol突变体的高通 ...

DNA analysis is predicting the likelihood of age-related conditions such as heart disease and cancer.