Piwowarek KŁ; Rzeszotarska A; Korsak JŁ; Juszkiewicz A; Chciałowski A; Kruszewski J INTRODUCTION: Platelet-activating factor (PAF) has a direct role as a mediator in the pathogenesis of various ...
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重组蛋白表达的原理: 重组蛋白表达是利用DNA重组技术,将目标基因(外源基因)导入宿主细胞中,并通过宿主细胞的生物机制使其表达出特定蛋白。其主要步骤包括: 基因克隆:将目标基因经过PCR扩增后,与表达载体连接,形成重组质粒。 转染或转化:将 ...