近日，北京大学化学与分子工程学院本科校友、美国威斯康星麦迪逊大学博士毕业生、目前正在斯坦福大学从事博士后研究的朱彦宇和所在团队研发出一种高时空分辨率的活细胞 DNA 成像技术 Oligo-LiveFISH。该技术基于 sgRNA ...

A research team has developed a microbubble-based nano-gene delivery platform (BTN MB) that precisely delivers gene ...

When the amount of cholesterol in the blood is too high, hypercholesterolemia can develop, causing serious damage to the arteries and cardiovascular ...

Standard DNA oligos, synthesized through phosphoramidite synthesis—historically considered the gold standard of DNA synthesis—are typically up to 100 bases (but can go up to 300 bases).

Introduction. Phosphoramidite solid-phase chemical DNA synthesis (CDS) (Beaucage and Caruthers, 1981) is the standard method for producing de novo single-stranded DNA (ssDNA) and is used on a large ...

There are plenty of companies making everything from short DNA fragments (called “oligos”) ... Phosphoramidite chemistry, developed in the 1980s, is the standard method for DNA oligo synthesis.

The gold standard for DNA writing, phosphoramidite chemistry, works incredibly well on short, uncomplicated, oligos. For longer stretches of DNA, which may contain DNA with complex features such ...

A nearest neighbor model is used to calculate melting temperatures along with Santa Lucia’s thermodynamic parameters (Santa Lucia and Hicks, 2004), the salt and oligonucleotide concentrations, and the ...