We constructed a modified form of the VS ribozyme containing an imidazole ring in place of adenine at position 756. The novel ribozyme is active in both cleavage and ligation reactions. The reaction ...

Methods and Results— We describe a novel approach to inhibiting the expression of the TF protein that involves the targeted destruction of cellular TF mRNA with the use of a tetraloop hairpin ribozyme ...

The hammerhead ribozyme is a well-studied nucleolytic ribozyme that catalyzes the self-cleavage of the RNA phosphodiester backbone. Despite exptl. and theor. efforts, there remain key questions about ...

For ribozyme delivery, 1×10 5 quiescent cells were incubated for 6 hours with 2.5 μg of chimeric ribozyme complexed with 10 μg of LipofectAMINE, according to the manufacturer’s suggestions. For ...

Camurus licensed its FluidCrystal drug delivery tech to Eli Lilly for global development of long-acting incretin therapies targeting metabolic diseases.

Top biotech M&As of May 2025. In April, the major pharma giants in the mergers and acquisitions (M&As) space were Novartis and Merck. Last month, however, the big names that announced ...

为解决 rna 复制中 “链分离问题”，研究人员开展 rna 聚合酶核酶（tpr）催化 rna 复制研究。发现三核苷酸三磷酸（pppnnn）可稳定单链 rna，结合 ph - 冻融循环实现双链 rna 指数复制，还使随机序列池向原始密码子漂移，为模拟原始 rna 复制提供新路径。

More information: James Attwater et al, Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme, Nature Chemistry (2025 ...

Trinucleotide substrates under pH–freeze–thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme. Nature Chemistry, 2025; DOI: 10.1038/s41557-025-01830-y; ...

Chemists at UCL and the MRC Laboratory of Molecular Biology have demonstrated how RNA (ribonucleic acid) might have replicated itself on early Earth – a key process in the origin of life.