CRISPR-Cas9 genome editing exploits the CRISPR-Cas system to modify a genome in a targeted manner. Guided by RNA, the Cas9 endonuclease breaks DNA at a target sequence. Imprecise repair of the ...

Conventional methods: High effort and limited spectrum CRISPR screening methods can be used to systematically examine genes for their function in cells. "CRISPR is used to switch off a random gene ...

金秋十月，Nature Methods杂志迎来了自己的第十次生日，编辑们为此选出了近十年来最受人瞩目的十大技术。二代测序、细胞重编程、光遗传学、CRISPR等技术就像一次次海啸，席卷了生命科学的各个角落，其中超高分辨率显微技术更是拿下了本年度的诺贝尔化学奖。

More information: Yuqing Mao et al, Enhanced detection for antibiotic resistance genes in wastewater samples using a CRISPR-enriched metagenomic method, Water Research (2024).

Since He Jiankui presented his results at last week’s gene editing summit, researchers have raised concerns about his protocol, calling the procedure “amateurish” and “unconscionable.” After a year ...

CRISPR is a technology that can be used to edit genes and, as such, will likely change the world. The essence of CRISPR is simple: it’s a way of finding a specific bit of DNA inside a cell.