Lentiviral systems for gene delivery are generally derived from the human immunodeficiency virus. The VDL utilizes a vector construct that employs a nonhomologous viral envelope (derived from the ...

Lentiviral vectors produced with accessory ... coprecipitation method 26: 7 μg envelope plasmid pMD.G, 13 μg packaging plasmid pCMVΔR8.74 or pCMVΔR8.2 and 20 μg transfer plasmid ...

The ‘transfer plasmid’ or lentiviral vector contains the transgene of interest, while the ‘envelope plasmid’ contains genes for proteins that appear on the virus surface, the ‘packaging ...

They can employ either recombinant viruses, such as adenovirus, adeno-associated virus (AAV), retrovirus, or lentivirus, or non-viral platforms, such as lipid nanoparticles, to transport the chosen ...