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Nature9y
Regulated selection of germinal-center cells into the memory B cell compartment
NP-specific IgG1 + LZ GC B cells from spleens of C57BL ... and then the EdU + cells were identified using a Click-iT EdU Pacific Blue Flow Cytometry Assay kit (Life Technologies) as instructed ...
Medscape2mon
Quantification of Nanoparticles at the Single-cell Level: An Overview About State-of-the-art Techniques and Their Limitations
Nanomedicine. 2014;9(12):1885-1900. While not specific to determining the precise localization of NPs, rather confocal and transmission electron microscopy are pertinent, flow cytometry can be ...
Nature19y
Transcription factor IRF4 controls plasma cell differentiation and class-switch recombination
For enrichment for NP-binding B cells in flow cytometry by 'gating out' of non–class-switched cells, CD19 + cell suspensions enriched by magnetic-activated cell sorting were stained with ...
Medscape2mon
Quantification of Nanoparticles at the Single-cell Level: An Overview About State-of-the-art Techniques and Their Limitations
These spectroscopy methods do, however, not give any information about the spatial localization of the NP in cells. Fluorescence-based quantification techniques Flow cytometry is a high-throughput ...