Department of Biophysical Chemistry, Lund University, Chemical Center, S-221 00 Lund, Sweden, Department of Laboratory Medicine, Division of Medical Microbiology, Lund University, Sölvegatan 23, S-223 ...

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利用通用引物对 338F 和 806R 扩增 16S rRNA 基因的 V3-V4 hypervariable region，经 Illumina MiSeq 系统进行高通量测序；最后，通过 QIIME、UCHIME、Vsearch 等软件对测序数据进行质量过滤、嵌合体去除和 OTU 聚类分析，并结合统计方法比较不同年龄组的微生物多样性和组成差异。

This valuable study reports a potential connection between the seminal microbiome and sperm quality/male fertility. The data are generally convincing. This study will be of interest to clinicians and ...