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钙调神经磷酸酶响应转录因子 Crz1 调控酵母 CMK2 基因表达的关键机制
在构建各种质粒方面,他们精心操作,构建出用于研究的质粒,如 CMK2p - lacZ 报告基因质粒等。蛋白质纯化技术也派上了用场,通过诱导表达和纯化 His6 - Crz1(N339 - S678)蛋白,为后续实验做准备。而电泳迁移率变动分析(EMSA)和染色质免疫沉淀(ChIP)实验则是 ...
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