Gene editing could be used to treat millions of glaucoma patients. Glaucoma is a leading cause of blindness, characterized by ...

pAAV-MCS Expression Vector of the AAV5 CMV Expression System kit (VPK-410-SER5, Cell Biolabs, United States) was used for construction of the pAAV-GFP vector to produce AAV5-GFP. The PCR product, ...

(C-D) Cultured EEN1 fl/fl hippocampal neurons were co-transfected with constructs for GFP or GFP-2A-Cre and Flag vector or Flag-endophilin A1 (Flag-EEN1) on DIV12, and immunostained for surface γ2 on ...

we constructed a 3'UTR sequence derived from TVMV and inserted it upstream of green fluorescent protein (GFP), resulting in the generation of a pEAQ-3'UTR-GFP expression vector. This was accomplished ...

The coding DNA sequence (CDS) of Cre in the pCAG-Cre vector was replaced by H2B-GFP from the Rosa-H2B-GFP mouse strain to construct the PB-pCAG-H2B-GFP vector. The inverted terminal repeat (ITR) ...

Source data are available online for this figure. Distribution of microglia by age, with subpanels separating the cells by treatment (PHP.GFAP‐IL2 or PHP.GFAP‐GFP control vector). C. Proportions of ...

Royalty-free licenses let you pay once to use copyrighted images and video clips in personal and commercial projects on an ongoing basis without requiring additional payments each time you use that ...

Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells.pMXs-IRES-GFP retroviral vector (also known as pMXs-IG) is based on Moloney murine leukemia virus ...

Then, the constructed vector was injected into y, sc, v, nanos integrase; attP40 stock and the transgenic line was screened following standard procedure. The following primary antibodies were used: ...

GFP has a potential use in the study of bacterial pathogenesis. The host–parasite interaction of three bacterial pathogens Salmonella typhimurium, Yersinia pseudotuberculosis, and Mycobacterium ...

Abstract: The distinct motion of GFP-tagged histone expressing cells (Histone-GFP type cells) has been investigated under ac electrokinetics in an electrode-multilayered microfluidic device as ...