we employed flow cytometry and fluorescence-activated sorting to purify the nuclei from different cell types.” The team simultaneously sorted GFP-positive and GFP-negative nuclei and used ...

Standard flow cytometry (FacsCalibur ... Unstained cells or cells incubated with GFP were used as controls. Two different protein array formats were tested. For the reverse array, 50–75 μl ...

The principle of flow cytometry is the segregation and analysis ... For Green channel on FL1 - Alexa Fluor 488, GFP, FITC, CFSE, DyLight 488 or CFDA-SE is used For Orange channel on FL2 ...

Flow cytometry provides a technique for obtaining information about ... This contains 4 lasers and 16 optical parameters, allowing detection of multiple fluorescent reporters (e.g. CFP, GFP, mRFP, ...

Functional monocyte flow cytometry offers an alternative to diagnosing ... that were engineered to express a green fluorescent protein (GFP), generating VSV-GFP. In response to IFN-a stimulation ...

A proof-of-principle experiment is shown in which both high and low green fluorescent protein (GFP)-expressing cells ... This method allows flow cytometry to be applied to RNA detection enabling ...

Flow cytometry is a powerful technique for the analysis of multiple parameters of individual cells within heterogeneous populations. Flow cytometry is used in a broad range of applications including ...

Flow cytometry is a widely used analytical technique that distinguishes between cell populations depending on the presence or absence of chosen markers. However, the number of markers that can be ...

The study, which used flow cytometry rather than molecular biology to identify circulating tumor cells (CTCs), found a ...

The BDFACSCelesta Flow Cytometer multicolor analyzer allows detection of up to 14 parameters using 3 lasers (405 nm, 488 nm, and 561 nm). The system has three preset flow rates of LO (12 uL/min), MED ...

The University's flow cytometry facility is a core facility based in the Royal Hallamshire Hospital and provides a service to all at the University and externally. Flow cytometry is a technique that ...