通过测量发光值，可量化药物处理、信号通路激活等对基因转录的影响。 双报告系统的协同作用： 为排除转染效率差异的干扰，试剂盒常整合 海肾荧光素酶（Renilla luciferase） 作为内参： 萤火虫荧光素酶检测目标基因活性，海肾荧光素酶（底物为腔肠素 ...

双报告系统：与海肾荧光素酶（Renilla Luciferase）联用，校正转染效率（如Dual Luciferase Reporter Assay Kit）。 高通量筛选（HTS） 稳态发光特性（信号持续数分钟）适配自动化检测，用于药物筛选或启动子活性分析。 体内成像 重组酶与底物D-荧光素钠（CAS 103404-75-7 ...

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BCA assay kits offer researchers a reliable and simple way to determine the protein concentration in a test sample. In this blog, we are going to explore how BCA protein assay kits function.

Toward this goal, we describe a solution phase split-luciferase assay for native protein detection, where we fused fragmented halves of firefly luciferase to separate receptor fragments or ...

Department of Pharmacology and Molecular Sciences, Johns Hopkins University, Baltimore, United States ...