Wild-type and mutant alleles were expressed in mammalian cells, which were homogenized and subjected to an iodotyrosine deiodinase assay for both monoiodotyrosine and diiodotyrosine substrates ...

The procedure summarized below, and which involved the synthesis of N-acetyl-tyrosine and N-acetyl-3 : 5-diiodotyrosine as intermediates, was finally adopted. All compounds were hence labelled in ...

Although the 3: 5-diiodotyrosine also present in the ‘colloid’ must take part in the activity, as this is proportional not to total thyroxine but to total iodine, the two di-peptides made from ...