精确扩增对于采用SYBR Green I技术的RT-qPCR的成功至关重要，因为这种染料会结合扩增过程中产生的所有双链DNA。这种一步法试剂含有超纯的AccuStart热 ...

表观遗传修饰在多种生物和病理过程中起着关键作用，其中5-甲基胞嘧啶（5mC）被认为是肿瘤发生和进展的早期分子事件，并已成为一个非常有前景的生物标志物。近年来，5-羟甲基胞嘧啶（5hmC）作为5mC的氧化产物，在正常哺乳动物生长和发育中也扮演着重要角色，并与癌症和神经疾病等疾病相关，显示出作为癌症诊断理想生物标志物的巨大潜力。然而，由于5hmC水平比5mC低约14倍，且其结构与C和5mC相似，使得5 ...

澳大利亚药品管理局澄清 ...

With the ever-growing popularity of quantitative PCR (QPCR) as a sensitive technique for quantifying DNA and RNA, there is increasing demand for higher throughput of reactions and faster protocol ...

目前，除通过有创的肝穿刺活检标本中留取标本进行HBV cccDNA的检测外，还不能完全依靠血液学的方法直接测HBV cccDNA水平。而患者对肝活检技术接受度低，迫使临床使用无创的替代指标来间接推测肝内HBV ...

qPCR, also known as real-time PCR, is an extension of traditional PCR, where DNA amplification over time is quantitated by measuring fluorescence from dyes intercalating into nascent DNA strands or by ...

（EvaGreen™ 完整的安全性报告可以从Biotium网站上下载） EvaGreen ® 是一种用于实时定量 PCR（qPCR）的 DNA 结合染料。该染料的诸多优点使它远胜于 SYBR Green I。除了有相似的光谱特性，EvaGreen 有三个主要特点使它区别于 SYBR Green I。 首先，EvaGreen 对 PCR 的抑制性远 ...

The heart of RT-qPCR is polymerase chain reaction (PCR), a well-established molecular technique that allows researchers to make millions of copies of a single DNA or RNA molecule. Traditional PCR ...

其中，抗体热启动修饰的 MonAmp™ SYBR® Green qPCR Mix，其核心组分是经抗体修饰的热启动 Taq DNA 聚合酶，配合精心优化的 Buffer 体系，有效保证扩增的高效率和高特异性，可对宽广浓度范围的模板进行准确定量，获得稳定可靠的qPCR 结果。 产品特点 高特异性 抗体热 ...

Among the methods of detecting residual DNA, qPCR is the most widely used for residual DNA quantitation due to its sensitivity, accuracy, precision, and time-saving capability. In this webinar, we ...

BHQnova ™ Probes are double-quenched hydrolysis probes with enhanced quenching and greater signal-to-noise ratio for long probes (>25 bases). Such probes consist of a 5' fluorophore dye, a 3 ...

Comet assay and western blot of γ-H2AX expression were performed to assess DNA damage occurring in HCC cells. RT-qPCR and western blot were used to identify the As2O3-targeted signals in macrophages, ...