The second component is TadA8e, an adenine deaminase for facilitating A-to-G conversion. The third component, DddA tox, is a cytosine deaminase that makes the DNA more accessible to TadA8e.

With the discovery of DddA-derived cytosine base editors ... show how integration of our mitochondrial cytosine base editor and the deaminase enzyme we evolved for our nuclear adenine base ...

Recently, a new tool for mitochondrial DNA mutagenesis has emerged—the double-stranded DNA deaminase (DddA)-derived cytosine base editor (DdCBE). A new study tested the base editor for in vivo ...

Programmable deaminases, which consist of a custom DNA-binding protein and a nucleobase deaminase ... genome editing: cytosine base editors and adenine base editors, such as DddA-derived cytosine ...

that can efficiently deaminate cytosine in DC context in double-stranded DNA (dsDNA). Their findings were published on February 16, 2023 in Nature Communications, with the title "DddA homolog ...

Mitochondrial diseases are a heterogeneous group of disorders with varying clinical features caused by impaired function of the mitochondrial respiratory chain. The underlying cause can be gene ...

Guide RNAs direct the CBE—which includes nuclease-free Cas9, cytosine deaminase, and uracil glycosylase inhibitor (to prevent uracil removal)—or ABE, which consists of nuclease-free Cas9 and adenosine ...

In one embodiment of the invention, a fusion protein was designed with both anti-epidermal growth factor receptor (EGFR) affibody and the prodrug enzyme cytosine deaminase, which can convert prodrug 5 ...