Introduction: Mitochondrial diseases caused by mtDNA have no effective cures. Recently developed DddA-derived cytosine base editors (DdCBEs) have potential therapeutic implications in rescuing the ...

Programmable deaminases, which consist of a custom DNA-binding protein and a nucleobase deaminase ... genome editing: cytosine base editors and adenine base editors, such as DddA-derived cytosine ...

By fusing a nickase Cas9 with ssDNA-specific deaminases, they have successively developed the cytosine base editor ... identified dsDNA-specific deaminase, DddA tox, to develop DdCBE, which ...

that can efficiently deaminate cytosine in DC context in double-stranded DNA (dsDNA). Their findings were published on February 16, 2023 in Nature Communications, with the title "DddA homolog ...

These molecular tools are fusions of a transcription activator-like effector domain (TALE), split-DddA deaminase halves, and an uracil glycosylase inhibitor (UGI) that have the distinct ability to ...