The cell supernatants containing [35 S]cysteine/methionine-labeled gp120 ... in binding buffer (50 mM HEPES-NaOH (pH 7.5), 2% dialyzed FCS (Gibco BRL, Grand Island, NY), 1 mM CaCl 2, 5 mM MgCl ...

The pH optimum of these reactions is 5–6, values that cannot be achieved ... this enzyme converts a critical cysteine residue to formylglycine (bearing an aldehyde at the C-α position) within ...

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The plasmids encoding His-GFP, His-TrxA, GST, MBP, GST-GFP, GST-TrxA, MBP-TvaF (a cysteine decarboxylase), and MBP-RPA2 (replication protein A2 ... The mixture was heated at 42°C for 45 s. and then ...