腔肠素400a（Coelenterazine 400a，CTZ 400a），常称为DeepBlue C，是一种天然腔肠素衍生物，是一款良好的海肾荧光素酶底物，但不适合用作分泌型膜锚定荧光素酶底物。 可用来实时监测钙离子浓度，报告基因分析，以及用作生物发光共振能量转移的底物。

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Cells were counted using a TC20 automated cell counter (Bio-Rad, Hercules, CA) and further diluted to 500,000 cells ml –1 in HBSS with 1 mM ascorbic acid and 2 μM of the nanoluciferase substrate ...

After the addition of MLT, the cells were incubated for 10 min at 37 °C, and coelenterazine 400a (2.5 μM; NanoLight Technology) was added 5 min before BRET reading in a Synergy Neo Microplate reader ...

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Mitochondrial Ca 2+ signal was evaluated 48 h after transfection of M8-CTL and M8-KD cells with adenoviral mutated mitochondria-targeted aequorin probe (mtAEQmut). mtAEQ was reconstituted with 5 μM ...

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The mitochondrial calcium uniporter channel (MCUC) mediates mitochondrial calcium entry, regulating energy metabolism and cell death. Although several MCUC components have been identified, the ...

The Animal Research Facility located in the ABSL-3 facility of the vivarium in the Saint Louis University Doisy Research Center offers sophisticated equipment for the optical imaging of small animals.