To synthesize large plasmid libraries, a high transformation efficiency is required. In contrast to cloning-based library synthesis methods, plasmid recombineering benefits from the transformation of ...

Specifically, we engineered a BstXI-based shotgun cloning (BSG) system, which consists of three entry vectors with siRNA expression units (SiEUs) flanked with distinct BstXI sites, and a retroviral ...

Duchenne muscular dystrophy (DMD) is a life-limiting X-linked recessive disorder and the most common genetic muscle disease, affecting approximately 1 in 3,500 live male births [1]. It is caused by ...