DNA immunoprecipitation-sequencing (DIP-seq). 5-methylcytosine (5mC). 5-hydroxymethylcytosine (5hmC). 5-formylcytosine (5fC). 5-carboxylcytosine (5caC). Nucleosome positioning and Open Chromatin ...

The CUT&Tag-IT R-loop Assay Kit is designed to be faster and more efficient than DNA:RNA immunoprecipitation (DRIP) and chromatin immunoprecipitation (ChIP) methods, and is compatible with 200,000 to ...

Real-time PCR was conducted using SYBR Green following cDNA synthesis. β-actin was used as an internal control. Chromatin immunoprecipitation (ChIP) assays (Millipore, 17-408) were carried out per the ...

Locusts, as devastating pests, can reversibly transform between solitary individuals and gregarious swarms with markedly different behaviors. Epigenetic regulation orchestrated by changes in chromatin ...

Samples were sequenced to an average target depth of 450 million reads per library. For identification of chromatin loop interactions, we used the KR-corrected 5 kb and 10 kb bin-sized cooler files ...

Objective To determine the molecular function of CCRK in HBV-associated HCC. Design Transcriptional regulation was assessed by chromatin immunoprecipitation, promoter mutation and luciferase reporter ...

The underlying mechanisms of metabolic and structural coupling were investigated by stable-isotope tracing metabolomics, coimmunoprecipitation coupled with mass spectrometry, and chromatin ...

5,6 Another option is methylated DNA immunoprecipitation sequencing (MeDIP-seq), an affinity-based ... highlighting its potential for innovative studies of 5-methylcytosine in chromatin regulation. A ...

Correspondence to Dr Susan M Farrington, CRUK Edinburgh Centre, Institute of Genetics and Cancer, The University of Edinburgh, Edinburgh, Edinburgh, UK; susan.farrington{at}ed.ac.uk Objective ...