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生物通
3 天
李属坏死环斑病毒RT-RAA-CRISPR/Cas12a可视化检测方法的建立与应用
摘要: 【目的】利用逆转录重组酶介导的核酸等温扩增技术(reverse transcriptase recombinase-aided amplification,RT-RAA)结合CRISPR/Cas12a ...
生物通
3 天
CRISPR-Cas12a小型crRNA库增强系统:汉坦病毒M基因超灵敏即时检测新策略
本研究针对CRISPR-Cas12a诊断系统存在的灵敏度不足、交叉污染风险及扩增-检测步骤不兼容等问题,开发了小型crRNA库增强平台 ...
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