BAC DNA could be purified with this kit using either an alcohol-precipitation protocol or a column-based protocol, depending on the volume of culture input and/or downstream application requirement.

BAC DNA probes were labeled using Alexa flour dyes (488, green and 594, red) (ThermoFisher). The BAC probes were labeled with biotin 14-dATP by nick translation (Gibco).

EPICENTRE has introduced BACMAX™ DNA Purification Kit that can enable isolation of exceptionally pure intact BAC DNA without binding columns, resins or organic extractions. The BAC DNA is suitable for ...