以ChIP-seq、CUT&Tag、CUT&RUN和ATAC-seq为代表的二代测序技术 ... 一. Spike-in引入 为了解决这一问题，研究人员提出了一种叫做加标Spike-in的方法。Spike-in最早是在RNA-seq技术中引入并广泛应用的。它的出现主要是为了解决RNA-seq数据分析中的标准化和定量问题。

Cyclin-CDK-defective phosphomimetic mutant of p27 (p27CK-DD) was overexpressed in 231 cells and p27 was also knocked down in 1833 cells. Combined ATAC-seq, RNA-seq, and p27 ChIP-seq showed p27 ...