Also, analyze the products of the small-scale reaction by alkaline agarose gel electrophoresis and store ... the desired volume of TE (pH 7.6). This protocol was adapted from “Stage 1: Synthesis ...

according to the manufacturer’s protocol. 20 µg/ml (200 ng) of DNA was incubated with increasing concentrations (10–200 ng) of proteins (thrombin, prothrombin) or peptides (GKY25, IVE25, or LL-37) in ...

16S Library was constructed according to the “16S Metagenomic Sequencing Library Preparation protocol” recommended by Illumina ... From each reaction, 5 μl were analyzed by 2% agarose gel ...