(c) Representative Western blots of phosphorylation levels of PKCɛ substrates, PKCɛ and GAPDH for HEK293T cells transfected with constructs listed. These cells were either subjected to 5 minutes of ...

We transiently transfected 293T cells in culture dishes (15-cm diameter) with pCMV-Myc-Dnmt1 (20 μg) or pcDNA3-HDAC1-F (10 μg) and collected cells 36 h post-transfection. We lysed cells in IPH ...

Mar. 11, 2025 — Researchers have identified genetic changes in blood stem cells from frequent blood donors that support the production of new, non-cancerous cells. Understanding the differences ...

This study aimed to explore the underlying mechanism by which PGE2 inhibits GSIS. Our results showed that PGE2 inhibited Kv2.2 channels via increasing PKA activity in HEK293T cells overexpressed with ...

Cellular unjamming is the collective fluidization of cell motion and has been linked to many biological processes, including development, wound repair, and tumor growth. In tumor growth, the ...

Your body is made of cells -- trillions of them. Cells are the basic building blocks of people and all other living things. Researchers study them to learn more about health and diseases.

Hu-MOG and Hu-P42S plasmids were transiently transfected into HEK293T cells using Lipofectamine2000 (Thermo Scientific, Carisbad, CA, USA). HEK293T cells were blocked with goat serum and ...

Stable RALY-overexpressed HEK293T cells were collected and total RNA was obtained by re-suspending and dispersing the cell pellet in complete RIP Lysis Buffer. Antibodies of RALY (ab170105, Abcam) or ...

Mar. 26, 2025 — Researchers have developed 'tomoseqr' -- a new software tool that enables easy estimation of the three-dimensional (3D) spatial distribution of gene expression. Tomoseqr is free ...

PARP7 is a mono-ADP-ribosyl transferase (mono-ART) which has recently gained attention due to its emerging role as a negative ...