MHC tetramers are typically prepared with most of the commonly used fluorescent labels used for flow cytometry. One major advantage of the technique is that the label is already attached to the ...

On occasion, it may be advantageous to use tetramers conjugated with APC, a more expensive fluorophore, which increases the cost of the tetramer to $900. The yield from a tetramer preparation is ...

We recommend you investigate staining at 4°C for 30-60 minutes, or room temperature for 30 minutes. Some tetramers have been observed to “fall apart” at 37°C. Titer your reagents before performing a ...