Normally, the CCA-adding enzyme adds CCA to the 3’ ends of tRNAs, a critical step in tRNA biogenesis that generates the amino acid attachment site ... the transcript is efficiently translated in vivo ...

Prominent features of the tRNA structure, as shown in the figure, include: an acceptor stem with the CCA trinucleotide at the 3' end (the site of aminoacylation and trans-peptidation reactions in ...